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KMID : 1094020180350050195
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Journal of Veterinary Clinics
2018 Volume.35 No. 5 p.195 ~ p.199
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Zinc Increases Chemotactic Activity of Porcine Peripheral Blood Polymorphonuclear Cells
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Seo Dong-Hee
Kang Byeong-Teck
Kang Ji-Houn
Yang Mhan-Pyo
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Abstract
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Zinc is necessary for normal functions in the immune system. The objective of the study is to examinethe effect of zinc on the chemotactic activity of porcine peripheral blood polymorphonuclear cells (PMNs). A modifiedBoyden chamber was used to determine the directional migration distance of PMNs. Various concentrations of zincshowed no chemotactic activity to PMNs. However, culture supernatant from peripheral blood mononuclear cells(PBMCs) treated with zinc remarkably increased the chemotactic activity of PMNs when compared with culturesupernatant from PBMCs treated without zinc. Culture supernatant from PBMCs treated without zinc also increasedthe migration distance of PMNs relative to vehicle control (medium alone). Increasing effect in chemotactic activityof PMNs by culture supernatant from PBMCs treated with zinc was inhibited by treatment of porcine anti-interleukin(IL)-8 polyclonal antibody (pAb). This effect was not affected by heat treatment (4-85oC). This corresponded withheat stable physical characteristics of IL-8. These results suggest that zinc can upregulate the chemotaxis of PMNs,which is primary mediated by IL-8 chemotactic factor released from PBMCs treated with zinc.
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KEYWORD
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zinc, chemotaxis, peripheral blood mononuclear cells, peripheral blood polymorphonuclear cells, IL-8, pig
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